fa فیزیولوژی ورزشی Sport Physiology تحقيقي Original Paper <div><strong>ABSTRACT</strong><br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and objectives</strong><strong><span dir="RTL">:</span></strong> Pterygium is a non-cancerous growth of conjunctival tissue that can extend onto the corneal surface. The presence of some oncogenic viruses in pterygium and the neoplastic nature of these lesions led us to the postulated involvement of the viruses in the etiology of pterygium. Given the <em>association of human herpesvirus 6 (HHV-6) with ocular diseases, we aimed to </em>investigate <em>presence</em> of this virus in <em>pterygium.</em><br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods: </strong>Fifty tissue specimens were collected from patients with pterygium who underwent pterygium surgery between February 2013 and May 2015. The specimens were tested by real-time PCR using <a href="https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0CBsQFjAA&url=https%3A%2F%2Fwww.lifetechnologies.com%2Forder%2Fcatalog%2Fproduct%2FK0221&ei=Ym9CVca3M4TyUJjsgfAO&usg=AFQjCNF4REjKcIXZu-Zc9VeDBDvi02v2lA&bvm=bv.92189499,d.ZWU">Maxima SYBR Green/ROX qPCR Master Mix (2X)</a> kit. Demographic and clinical data were collected and analyzed using SPSS software (version 18).<span dir="RTL"></span><br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results:</strong> Six (12 %) specimens were positive for HHV-6 DNA. There was no statistically significant correlation between pterygium and presence of HHV-6.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion: </strong>Based on the results, a direct association between HHV-6 and development of pterygium seems less probable, which suggests that other etiologic agents must be involved in the multistep process of the disease.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords: </strong>Human Herpesvirus 6; pterygium; Real-time PCR.</div> Human Herpesvirus 6, pterygium, Real-time PCR. 16 22 http://mlj.goums.ac.ir/browse.php?a_code=A-10-623-4&slc_lang=fa&sid=1 Mishar Kelishadi 100319475328460018836 100319475328460018836 No Laboratory Sciences Research Center, Golestan University of Medical Science, Gorgan, Iran Department of Virology, Pasteur Institute of Iran, Tehran, Iran Mandana Kelishadi 100319475328460018837 100319475328460018837 No Department of Ophthalmology, 5th Azar Hospital, Gorgan, Iran Akramsadat Ahmadi 100319475328460018838 100319475328460018838 No Laboratory Sciences Research Center, Golestan University of Medical Science, Gorgan, Iran Naeme Javid 100319475328460018839 100319475328460018839 No Laboratory Sciences Research Center, Golestan University of Medical Science, Gorgan, Iran G.Hossein Ashrafi 100319475328460018840 100319475328460018840 No Kingston University London, Cancer Theme, School of Life Science, Pharmacy and Chemistry, SEC Faculty, Kingston upon Thames, KT12EE, London, UK Alijan Tabarraei alijant@yahoo.com 100319475328460018841 100319475328460018841 Yes Infectious Diseases Research Center، Golestan University of Medical Sciences, Gorgan, Iran