fa تحقيقي Original Paper <div><strong>ABSTRACT</strong><br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objective:</strong> Prophage sequences are major contributors to interstrain variations within the same bacterial species. <em>Acinetobacter baumannii</em> is a gram-negative bacterium that causes a wide range of nosocomial infections, especially in intensive care unit inpatients. Prophage sequences constitute a considerable proportion of several sequenced complete genomes of <em>A. baumannii</em>. The aim of this study was to investigate the presence of prophage sequences in <em>A. baumannii</em> strains isolated from burn patients, and compare the results with other studies.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods:</strong> Presence of eight prophage sequences was investigated in the genome of ten multi-drug resistant <em>A. baumannii</em> isolates obtained from burn sites of 10 burn patients in a hospital in Isfahan, Iran. PCR and sequencing were performed to detect the prophage sequences. The presence of the eight prophage sequences in the genome of <em>A. baumannii</em> strains from other studies was investigated by BLAST analysis of whole nucleotide sequence of prophage sequences.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results:</strong> The isolates in the present study had different prophage sequence profiles. Two isolates did not contain any of the sequences, while two isolates contained three and two of the prophage sequences. Other isolates contained only one sequence. The prophage sequence profiles observed in this study were not found in <em>A. baumannii</em> isolates from other studies.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion:</strong> The results of this study indicate that the prophage sequences profile can be useful for studying the epidemiology of <em>A. baumannii</em> strains.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords:</strong> <em>Acinetobacter baumannii</em>, genome, prophage sequences.</div> Acinetobacter baumannii, genome, prophage sequences. 35 41 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-462&slc_lang=fa&sid=1 Nima Shaykh Baygloo 100319475328460012424 100319475328460012424 No Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran Majid Bouzari bouzari@sci.ui.ac.ir 100319475328460012425 100319475328460012425 Yes Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran Fateh Rahimi 100319475328460012426 100319475328460012426 No Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran