fa تحقيقي Original Paper <p></p> <div> <p><strong>ABSTRACT </strong></p> <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <strong>Background</strong> <strong>and Objectives:</strong> &nbsp;Many species of &nbsp;<em>Ziziphora </em>have been used in traditional medicine in the Azarbaijan region, northwest of Iran. This study aimed to determine antibacterial activity of essential oil and methanol extract of <em>Ziziphora tenuior </em>on some pathogenic bacteria isolated from urogenital tract infections.</p> <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <strong>Methods:</strong> The essential oil and methanol extract of <em>Z</em><em>. </em><em>tenuior </em>were obtained by Clevenger and maceration methods. Under sterile conditions, the required amount of urine was taken from patients referred to a number of clinics in Tabriz during 2014. After identification of the isolates using standard microbiological methods, antimicrobial effects of the essential oil and methanol extract of the plant on the isolates were evaluated by determining minimum inhibitory concentration (MIC), minimum bactericidal concentration, and antibiogram test.</p> <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <strong>Results:</strong> The MIC for essential oil of <em>Z</em><em>. </em><em>tenuior</em> was 250&micro;g/mL for most Gram-negative bacteria except <em>Pseudomonas</em> <em>aeruginosa</em>. Moreover, the MIC for <em>Staphylococcus aureus</em> was 250&micro;g/mL and 500&micro;g/mL for other strains of Staphylococcus.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion:</strong> Comparison of the inhibitory and bactericidal effects of the essential oil and methanol extract of <em>Z</em><em>. </em><em>tenuior</em> showed that the essential oil is able to inhibit growth of the bacteria tested even in low concentrations. Further studies are required in this regard using animal models.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords: </strong>Antibacterial Agents, <em>Ziziphora tenuior</em>, Plant Extracts, Urinary Tract Infection.&nbsp;</p> </div> Antibacterial Agents, Ziziphora tenuior, Plant Extracts, Urinary Tract Infection. 54 59 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-434&slc_lang=fa&sid=1 Younes Anzabi 100319475328460012473 100319475328460012473 No Department of Pathobiology, Tabriz Branch Arash Khaki khaki@iaut.ac.ir 100319475328460012474 100319475328460012474 Yes Department of Pathobiology, Tabriz Branch