fa PCR تعین فراوانی یرسینیا انتروکلی تیکادر نمونه های اسهالی شهر گرگان با روش Abstract Background and objectives: After respiratory infection, Diarrhea is the second cause of mortality. Yersinia enterocolitica is the second important cause of infectious diarrhea in children of some countries. In this study, we evaluated the frequency of Yersinia entocolitica of diarrheal specimens in Gorgan, Iran. Material and Methods: This descriptive cross - sectional Study was carried out on diarrheal stools of 455 patients referred to medical centers and laboratory of Gorgan in 2004-2005. DNA extraction using phenol chloroform was performed for all samples. Using two specific primers (genus-specific16s rRNA and ail- specific species genus of Yersinia enterocolitica), we did PCR sample. Results: Yersinia genome was identified in 12 patients(2.63%) and 11 of them was Yersinia enterocolitica. The frequency infection in of girls (3%) was more than boys (2.4%), and the prevalence in winter (4%) was more them other seasons, and under one- year- group (3.4%) and 1-5 years (3.1%) is more than other age groups. It was not observed significant difference. (P> 0.05). Conclusion: The frequency of Yersinia in cases of diarrhea in Gorgan is similar to most regions of Iran and in children under 5 years is observed more in winter. Key words: Yersinia enterocolitica, Diarrhea, children, Gorgan Key words: Yersinia enterocolitica, Diarrhea, children, Gorgan 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-5&slc_lang=fa&sid=1 F Ghasemi Kebria فاطمه قاسمی کبریا kebria_fgh62@yahoo.com 100319475328460013281 100319475328460013281 Yes Microbiology, Golestan Research center of مرکز تحقیقاتگوارش B Khodabakhshi بهناز خدابخشی 100319475328460013282 100319475328460013282 No گوارشو کبد دانشگاه علوم پزشکی گلستانمرکز تحقیقات H Kouhsari هادی کوهساری 100319475328460013283 100319475328460013283 No دانشگاه آزاد اسلامی M Sadeghi Sheshpoli مریم صادق ششپلی 100319475328460013284 100319475328460013284 No و انکولوژی دانشگاه علوم پزشکی گلستان N Behnampoor ناصر بهنام پور 100319475328460013285 100319475328460013285 No مرکزتحقیقاتهماتولوژی S Livani صدیقه لیوانی 100319475328460013286 100319475328460013286 No مرکز تحقیقاتبیماریهای عفونی ، M Bazuori مسعود بازوری 100319475328460013287 100319475328460013287 No مرکز تحقیقاتبیماریهای عفونی ، دانشگاه E A Ghaemi عزت الله قائمی 100319475328460013288 100319475328460013288 No مرکز تحقیقات