fa ارزیابی IgG اختصاصی سرمی و پاسخ سایتوکینی لنفوسیت‌ های خون محیطی بیماران مبتلا به بروسلوز نسبت به پروتئین سطحی 31 کیلودالتون نوترکیب، یک کاندیدای واکسن بروسلا تحقيقي Original Paper Abstract Background and Objective: One of the proteins shared in all strains of Brucella is 31 kDa surface protein (BCPS31) that could be an appropriate target for immunization and serological diagnosis. Material and Methods: In the present study, BCSP31 produced as a recombinant protein in pET28a (+) expression system was utilized, using ELISA, to detect trace specific antibody (IgG) in brucellosis patients' serum that was confirmed by culture. We also evaluated cytokine response of peripheral blood lymphocytes to this protein in the cell culture. Results: The results indicated a significant amount of surface protein antibodies (IgG) in the serum of patients with brucellosis. Evaluation of lymphocyte responses to rBCSP31 also showed a significant IL-12 and IFN-γ production in patients’ lymphocyte cultures. Conclusion: These results suggest that BCSP31 can elicit specific humoral and cellular responses during host infection and it can be used in designing immunization and serologic diagnosis systems. Keywords: Brucellosis, 31kDa Cell Surface Protein, Brucella, Cytokine Brucellosis, 31kDa Cell Surface Protein, Brucella, Cytokine 36 42 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-267&slc_lang=fa&sid=1 N Khoramabadi نیما خرم‌آبادی 100319475328460012663 100319475328460012663 No دانشگاه تربیت مدرس، تهران A Mohabati Mobarez اشرف محبتی مبارز mmmobarez@modares.ac.ir 100319475328460012664 100319475328460012664 Yes دانشگاه تربیت مدرس، تهران H Aghababa هانیه آقابابا 100319475328460012665 100319475328460012665 No دانشگاه تربیت مدرس، تهران B Behmanesh مهرداد بهمنش 100319475328460012666 100319475328460012666 No دانشکده علوم ز، دانشگاه تربیت مدرس، تهران B Tabaraie بهمن تبرایی 100319475328460012667 100319475328460012667 No F Atyabi فاطمه اطیابی 100319475328460012668 100319475328460012668 No دانشگاه علوم پزشکی تهران