fa مقاومت آنتی بیوتیکی به سفتازیدیم و سفتریاکسون و ردیابی ژن TEM در سویه های اشریشیا کلی تحقيقي Original Paper <p>Abstract Background and Objective: In the past, most strains of E. coli were susceptible to a wide range of antimicrobial agents, but this situation is now changed by indiscriminate use of antibiotics. Ceftriaxone and Ceftazidime are the most current antibiotics used for Enterobacteriaceae infections in hospitals. The aim of this study was to determine antimicrobial resistance of Escherichia coli strains isolated from patients. Material and Methods: During a 12-month period, 200 clinical samples taken from patients referred to Zahedan hospitals were assessed to isolate Escherichia coli. Antibiotic susceptibility was determined by disk diffusion method and micro-broth dilution and Bla TEM resistance genes were detected by PCR. Results: Following phenotype verification testing, 112 isolates (56%) were produced Extended Spectrum Beta Lactamase (ESBLs) and 130 isolates were potential producers of beta-lactamase (ESBL). Using PCR, 72 isolates (38.55%) have TEM gene. Conclusion: The rate of antibiotic resistance of Escherichia coli isolates to ceftriaxone and ceftazidime is high therefore, it seems reasonable to do antibiogram before treatment. Keywords: Extended-Spectrum-Beta-Lactamase, Esherchia coli, Disc Diffusin, Micro-Broth Dilution</p> Extended-Spectrum-Beta-Lactamase, Esherchia coli, Disc Diffusin, Micro-Broth Dilution 20 26 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-252&slc_lang=fa&sid=1 Jahani, S سمیه جهانی s_jahani66@yahoo.com 100319475328460010969 100319475328460010969 Yes Falavarjan Branch, Falavarjan Isfahan, Iran دانشگاه آزاد اسلامی واحد فلاورجان، اصفهان Shahreki Zahedani, SH شهرام شهرکی زاهدانی 100319475328460010970 100319475328460010970 No Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran دانشگاه علوم پزشکی زاهدان Karbasizade, V وجیهه کرباسی زاده 100319475328460010971 100319475328460010971 No Falavarjan Branch, Falavarjan Isfahan, Iran دانشگاه آزاد اسلامی واحد فلاورجان، اصفهان