fa توزیع فراوانی کمپیلوباکترژژونی درنمونه های اسهالی شهرگرگان Abstract Background and Objectives: Diarrhea is one of the most common diseases in the world. Campylobacter jejuni is one of the prevalent agents of bacterial diarrhea in most of developing countries. It is usually ignored in routine laboratory test in our country, because it has a difficult investigation method. This article aims to determine the prevalence of Campylobacter jejuni, in diarrhea samples in Gorgan City (East north of Iran) by PCR Method. Material and Methods: This descriptive study was performed on 455 diarrheal samples during one year (2005-06).255 out of them were cultured on Preston media (Himedia co.) on 42°c. DNA Extracted by phenol cholorophorm method was directly carried out on stool samples.16srDNA hipo and asp primers for detection of Campylobacter genus, C.jejuni and C.coli species were used, respectively. In addition, universal primer of 16srDNA was used for control of PCR method. Results: no sample was positive for Campylobacter in culture .only three samples were positive for Campylobacter genus and C.jejuni specific primer but none of them were positive for C.coli .99 samples were positive by universal primer of 16srDNA . Conclusion: The results indicate that C.jejuni isn't a prevalent agent in diarrhea in our region. Key words: Campylobacter jejuni -Gorgan- Diarrhea Key words: Campylobacter jejuni -Gorgan- Diarrhea 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-39&slc_lang=fa&sid=1 N Ziaei ندا ضیائی neda.ziaee61@gmail.com 100319475328460013135 100319475328460013135 Yes Ms in Microbiology,Lahijan Azad دانشگاه آزاد اسلامی واحد لاهیجان N Amir Mozafari نور امیرمظفّری 100319475328460013136 100319475328460013136 No Associate professor in Molecular Biology, دانشگاه علوم پزشکی ایران H Kouhsari هادی کوهساری 100319475328460013137 100319475328460013137 No ،،دانشگاه آزاد اسلامی واحد آزادشهر A Moradi عبدالوهّاب مرادی 100319475328460013138 100319475328460013138 No دانشگاه علوم پزشکی گلستان A Tabarai علیجان تبرّائی 100319475328460013139 100319475328460013139 No دانشگاه علوم پزشکی گلستان T Dadgar تینا دادگر 100319475328460013140 100319475328460013140 No دانشگاه علوم پزشکی گلستان S Livani صدیقه لیوانی 100319475328460013141 100319475328460013141 No دانشگاه علوم پزشکی گلستان M Arab Ahmadi مریم عرب احمدی 100319475328460013142 100319475328460013142 No