fa جداسازی، شناسایی ملکولی و تعیین اثر ضد قارچی باسیلوس های جداشده از خاک ریزوسفریک منطقه گرگان علیه تریکوفیتون منتاگروفایتس تحقيقي Original Paper Abstract Background and objective: Soil bacteria, particularly Bacillus genus have the potential of producing a range of bioactive substances with antimicrobial and antifungal properties. They have the ability to produce hundreds of active and effective biologic compound against microorganisms. Therefore, it seems to be a proper candidate in the biocontrol of fungal pathogenesis. Material & Methods: In this study, soil samples were collected from different parts of Gorgan in order to isolate Bacillus and to determine their antifungal activity against T.mentagrophytes. The Isolates that had the highest antifungal effects were analyzed by PCR and 16s rRNA sequencing. Results: of 54 strains, 14 have antifungal activity. The Isolates, S4 and S12, identified as B.cereus and B.thuringiensis respectively show the highest antidermatofit effect. These isolates based on 16s rRNA sequence analysis show 97% homology with Bacillus cereusstrain KU4 and Bacillus thuringiensisstrain ucsc27. Conclusion: According to the results, it seems that the soil Bacilli have biocontrol potential against dermatophytic agents such as T.mentagrophytes. Keywords: Antifungal effects, Bacillus, Rhizospheric soil, T.mentagrophytes Antifungal effects, Bacillus, Rhizospheric soil, T.mentagrophytes 18 22 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-146&slc_lang=fa&sid=1 HR Pordeli حمیدرضا پردلی h_pordeli@yahoo.com 100319475328460012888 100319475328460012888 Yes Assistant Professor of Mycology, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Gorgan Branch دانشکده علوم پایه دانشگاه آزاد اسلامی واحد گرگان،گرگان،ایران SJ Hashemi Hazaveh سید جمال هاشمی هزاوه 100319475328460012889 100319475328460012889 No Associated Professor of Mycology, Department of Mycology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran دانشکده علوم دامپزشکی، M Jamshidian محمود جمشیدیان 100319475328460012890 100319475328460012890 No Professor of Microbiology, Department of Microbiology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran دانشکده علوم دامپزشکی M Bayat منصور بیات 100319475328460012891 100319475328460012891 No Associated Professor of Mycology, Department of Mycology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran دانشکده علوم دامپزشکی