Medical Laboratory Journal

en Detection and identification of Prevotella Melaninogenica in saliva samples of patients with ulcerative colitis ميکروب شناسي Microbiology Research Article Research Article Background: Ulcerative colitis (UC) is a long-term inflammatory bowel disease (IBD) caused by abnormal immune responses, leading to inflammation and scarring in the large intestine. The bacteria Prevotella melaninogenica, found in the intestine and mouth, may contribute to UC. This study focuses on the detection of P. melaninogenica in the saliva of UC patients and compares them with the healthy control (HC) group. Methods: The present study was a case-control study including 40 UC patients and 40 healthy controls (HCs) with an average age of 43.0 3± 10.3. This study used a real-time PCR test to investigate the frequency and average number of P. melaninogenica from the 16S rRNA gene sequence of P. melaninogenica in both groups. Results: P. melaninogenica was more frequent in UC patients (77.5%) than HCs (45%) (p = 0.003). The patient group had more bacteria (339.31 ± 1082.29) than HCs (61.29 ± 154.03) (p = 0.005). Women in the UC group had more P. melaninogenica (492.35 ± 1427.61) than the control group (56.98 ± 123.50) (p = 0.0342). Similarly, men in the UC group (262.85 ± 664.97) had more bacteria than the control group (72.62 ± 222.76) (p = 0.015). Conclusion: The current study showed that dysbiosis in P. melaninogenica, a bacterium in human saliva, could be important in the development of UC. Further investigation is needed to evaluate its use as a potential biomarker in the UC.   Colitis, Ulcerative, Prevotella melaninogenica, Saliva, Dysbiosis 26 28 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1531-1&slc_lang=en&sid=1 Samin Zamani samin.zamanii@gmail.com 100319475328460027452 100319475328460027452 No Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran;Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran Sima Besharat s_besharat_gp@yahoo.com 100319475328460027453 100319475328460027453 No Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran Nasser Behnampour behnampour@yahoo.com 100319475328460027454 100319475328460027454 No Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, Iran Armina Behnam ar_be96@yahoo.com 100319475328460027455 0009-0008-9879-3171 No Dental Research Center, Golestan University of Medical Sciences, Gorgan, Iran Negar Asgari asgarinegar4@gmail.com 100319475328460027456 100319475328460027456 No Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran Nazanin Mortazavi mortazavi_nazanin@yahoo.com 100319475328460027457 100319475328460027457 Yes Dental Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Oral and Maxillofacial Medicine, School of Dentistry, Golestan University of Medical Sciences, Gorgan, Iran