en ميکروب شناسي Microbiology Research Article Research Article <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Background and objectives:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> A</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">flatoxin B1</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> (AFB1) is the most toxic aflatoxin produced by a large number of <i>Aspergillus</i> species. Successful detoxification of this toxin is an important attempt to improve community health. The aim of this study was to evaluate reducing effects of yeasts isolates from kefir and traditional kefir-like fermented beverages on AFB1 in a </span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">broth medium</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Methods: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Polymerase chain reaction-sequencing was carried out to identify the yeast isolates from kefir <a name="_Hlk90058677">and kefir-like beverages</a>. Effects of the isolates on AFB1 adsorption and biotransformation in peptone dexterose broth medium were evaluated by using high performance liquid chromatography</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">. </span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b> <i><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Saccharomyces cerevisiae</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> and <a name="_Hlk94377706"><i>Kluyveromyces marxianus</i></a> were isolated from kefir and kefir-like beverages and resulted in </span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">46% and 53% AFB1 adsorption, respectively. The isolates 27Y and 2Y caused 7% toxin biotransformation, while 10% toxin biotransformation was achieved by the isolate 18Y.&nbsp; </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> <a name="_Hlk90131776">Our results indicate that the yeast isolates from kefir and traditional kefir-like products can bind to and detoxify AFB1, thereby reducing its harmful effects.</a></span></span></span></span></span></span> Kefir,Yeasts,Aflatoxin B1,Adsorption,Biotransformation, 20 25 http://mlj.goums.ac.ir/browse.php?a_code=A-10-641-2&slc_lang=en&sid=1 Abdoljalil Eiri jaliliri@gmail.com 100319475328460022789 100319475328460022789 No Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Hami kaboosi h.kaboosi@iauamol.ac.ir 100319475328460022790 100319475328460022790 Yes Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Farhad Niknejad fniknezhad@yahoo.com 100319475328460022791 100319475328460022791 No Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran and Department of Clinical Laboratory Sciences, Faculty of Para Medicine, Golestan University of Medical Sciences, Gorgan, Iran Abdollah Ardebili ardebili_abdollah57@yahoo.com 100319475328460022792 100319475328460022792 No Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran and Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Hamid Reza joshaghani hr_joshaghani@yahoo.com 100319475328460022793 100319475328460022793 No Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran and Department of Clinical Laboratory Sciences, Faculty of Para Medicine, Golestan University of Medical Sciences, Gorgan, Iran