fa فراوانی دی آنتامبا فراژیلیس با استفاده از دو روش رنگ آمیزی هماتوکسیلین آهن و Nested-PCR در مراجعین به مراکز درمانی چالوس Abstract Bachground and objectives: Dientamoeba fragilis is a habitant protozoa in human colon causing clinical symptoms, such as local stomach pain, weight loss, lack of appetite and flatulence. It is important to diagnose this infection correctly and differentiate it from other Protozoa. In this study PCR and Iron Hematoxylin methods were used to detection of this protozoa in Chalous Medical centers refers in 2010. Material and Methods: The stool samples (n=302) of this cross-sectional study were selected via cluster random sampling. After wet mount study the samples were preserved in PVA (for staining) and Ethanol (for molecular). The samples were studied both Staining and Molecular methods. Sensitivity and specificity were assessed. Results: Of 302 samples, six of them are positive via staining method (1.99%) and five by molecular method. All negative results with staining method are also negative with PCR. Contamination with E.coli in 2 samples and with Balstocystis homonis were seen in one sample. Sensitivity and specificity of PCR was 85% and 100% respectively. Conclusion: The discrepancy between two methods maybe caused by observer errors in staining method and unsynchronized molecular and microscopic studies. Key words: Dientamoeba fragilis, PCR, Iron Hematoxylin, Chalous region Key words: Dientamoeba fragilis, PCR, Iron Hematoxylin, Chalous region 1 6 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-86&slc_lang=fa&sid=1 S H Alizadeh Shargh شهره علیزاده شرق shohrehshargh@gmail.com 100319475328460013313 100319475328460013313 Yes FacultyMember of Dept of دانشگاه آزاد اسلامی واحد چالوس A Ghazanchaei اردوان قازانچائی 100319475328460013314 100319475328460013314 No دانشگاه علوم پزشکی تبریز A A Ayetollahi علی اصغر آیت الهی 100319475328460013315 100319475328460013315 No سرپرست دانشکده پیراپزشکی و بهداشت استان گلستان A Khandan Del ابولفضل خندان دل 100319475328460013316 100319475328460013316 No آزمایشگاه بیمارستان 5 آذر گرگان،علوم پزشکی گرگان B Pourasghari بهروز پوراصغری 100319475328460013317 100319475328460013317 No آزمایشگاه مرکز آموزشی درمانی و تحقیقاتی تبریز R Estakhri رسول استخری 100319475328460013318 100319475328460013318 No مرکز آموزشی درمانی و تحقیقاتی امام رضا(ع)، تبریز