fa تحقيقي Original Paper <strong>ABSTRACT</strong><br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objectives: Pseudomonas aeruginosa is a gram negative opportunistic pathogen and an important cause of wound infections and nosocomial infections. The purpose of this study was to study inhibitory effects of a new ointment prepared from medicinal plants against P. aeruginosa isolates.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods:</strong> In this study, an ointment called ZOUSH was prepared from mixing alcoholic extracts of Satureja khuzestaniea, Zataria multiflora, Mentha mozaffariani Jamzad, honey and polyurethane. Minimal inhibitory concentration of ZOUSH and its compositions alone or combined was determined using the disk diffusion method.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results: </strong>S. khuzestaniea, Z. multiflora and Mentha mozaffariani Jamzad had inhibitory effects against P. aeruginosa. The ZOUSH ointment had greater antibacterial effects than the any of its compositions used solely or combined. The diameter of inhibition zone had a direct relationship with the concentration of the extracts. Moreover, the antibacterial effect of the ZOUSH ointment was identical to that of polymyxine B (300 &micro;g).<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion:</strong> We demonstrated that the ZOUSH ointment has inhibitory effects against P. aerugionosa. The inhibition zone diameter is directly correlated with the concentration of the extracts. Our results suggest that the ointment could be useful for treatment of burn wounds and skin infections. Pseudomonas aeruginosa, ZOUSH, Burns, Satureja khuzestaniea, Zataria multiflora, Mentha Mozaffariani Jamzad, Honey, Polyurethane, Ointment 1 5 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-552&slc_lang=fa&sid=1 Maryam Meskini 100319475328460015150 100319475328460015150 Yes Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Azad Khaledi 100319475328460015151 100319475328460015151 No Department of Microbiology, Kashan University of Medical Science, Kashan, Iran Davoud Esmaeili esm114@gmail.com 100319475328460015152 100319475328460015152 No Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran, and Applied Virology Research Center, Baqiyatallah University of Medical Sciences